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Methods: Carbon tetrachloride (CCl4)treated mouse model in vivo and in hepatic stellate cells (HSC) in vitro were used. The effect on CCl4induced liver fibrosis was studied using histochemical and biochemical analysis, while the inhibition on HSC was assessed using cell proliferation/apoptosis assay and collagen I production using realtime PCR. The inhibitory effects of Leukamenin F on Akt/mTOR/p70S6K and TGF pathways was studied using Western blot and cell image analysis.
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